PRODUCT CODE: ET1704-40

Recombinant IKK gamma Monoclonal Antibody (ET1704-40)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of IKK gamma on Hela cell using anti-IKK gamma antibody at 1/1,000 dilution.
  • Western blot analysis of IKK gamma on Hela cell using anti-IKK gamma antibody at 1/1,000 dilution.
  • ICC staining IKK gamma in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with IKK gamma antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of IKK gamma on Hela cell using anti-IKK gamma antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IKK gamma Monoclonal Antibody (ET1704-40)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysates, Hela, human spleen tissue, human colon carcinoma tissue, mouse colon tissue, mouse fallopian tissue, human colon tissue, human placenta tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA11-83

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:20-1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

IKK gamma

SYNONYMS

IkB kinase associated protein 1 antibody; IkB kinase subunit gamma antibody; Inhibitor of nuclear factor kappa B kinase subunit gamma antibody; AMCBX1 antibody; FIP 3 antibody; FIP-3 antibody; FIP3 antibody; Fip3p antibody; I kappa B kinase gamma antibody; I-kappa-B kinase subunit gamma antibody; IkB kinase gamma subunit antibody; IkB kinase subunit gamma antibody; IkB kinase-associated protein 1 antibody; Ikbkg antibody; IKK-gamma antibody; IKKAP1 antibody; IKKG antibody; IMD33 antibody; Incontinentia pigmenti antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase gamma antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, gamma antibody; Inhibitor of nuclear factor kappa-B kinase subunit gamma antibody; IP antibody; IP1 antibody; IP2 antibody; IPD2 antibody; NEMO antibody; NEMO_HUMAN antibody; NF kappa B essential modifier antibody; NF kappa B essential modulator antibody; NF-kappa-B essential modifier antibody; NF-kappa-B essential modulator antibody; ZC2HC9 antibody

TISSUE SPECIFICITY

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-68 attenuates aminoterminal homodimerization.; Polyubiquitinated on Lys-285 through 'Lys-63'; the ubiquitination is mediated by NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-399 through 'Lys-63'; the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-277 and Lys-309; promotes nuclear export. Polyubiquitinated through 'Lys-27' by TRIM23; involved in antiviral innate and inflammatory responses. Linear polyubiquitinated on Lys-111, Lys-143, Lys-226, Lys-246, Lys-264, Lys-277, Lys-285, Lys-292, Lys-302, Lys-309 and Lys-326; the head-to-tail polyubiquitination is mediated by the LUBAC complex and plays a key role in NF-kappa-B activation. Deubiquitinated by USP10 in a TANK-dependent and -independent manner, leading to the negative regulation of NF-kappa-B signaling upon DNA damage.; Sumoylated on Lys-277 and Lys-309 with SUMO1; the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues.; Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity.; (Microbial infection) Cleaved by hepatitis A virus (HAV) protease 3C allowing the virus to disrupt the host innate immune signaling.; (Microbial infection) Polyubiquitinated on Lys-309 and Lys-321 via 'Lys-27'-linked ubiquitin by Shigella flexneri E3 ubiquitin-protein ligase ipah9.8, leading to its degradation by the proteasome.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.