Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lane 3: Daudi cell lysate
Recombinant Rabbit monoclonal primary
Recombinant IKK alpha + IKK beta Monoclonal Antibody (ET1611-23)
Recombinant protein within human ikk beta aa 460-660.
Hela cell lysate, A431 cell lysate, Daudi cell lysate, Hela, B16F1, C2C12.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
IKK alpha + IKK beta
CHUK antibody; Conserved helix loop helix ubiquitous kinase antibody; I kappa B kinase 1 antibody; I kappa B kinase 2 antibody; I Kappa B kinase alpha antibody; I Kappa B kinase beta antibody; IkB kinase alpha subunit antibody; IkBKA antibody; IkBKB antibody; IKK a kinase antibody; IKK alpha antibody; IKK beta antibody; IKK1 antibody; IKK2 antibody; IKKA antibody; IKKB antibody; IMD15 antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody; Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody; Inhibitor of nuclear factor kappa-B kinase subunit beta antibody; NFKBIKA antibody; NFKBIKB antibody; Nuclear factor NF kappa B inhibitor kinase beta antibody; Nuclear factor NFkappaB inhibitor kinase alpha antibody; TCF 16 antibody; TCF16 antibody; Transcription factor 16 antibody
Cytoplasm, Nucleus, Membrane raft.
The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase a (IKKa), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IkB-a on Ser 32 and 36, the sites that trigger its degradation. IKKa appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKa is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKa, IKKb and IKKg (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.
Cai, Guodong et al.
Mechanism and effects of Zearalenone on mouse T lymphocytes activation in vitro. | Ecotoxicology and Environmental Safety