PRODUCT CODE: ET1611-23

Recombinant IKK alpha + IKK beta Monoclonal Antibody (ET1611-23)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of IKK alpha + IKK beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: Hela cell lysate<br />
 Lane 2: A431 cell lysate<br />
 Lane 3: Daudi cell lysate
  • Western blot analysis of IKK alpha + IKK beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: Hela cell lysate<br />
 Lane 2: A431 cell lysate<br />
 Lane 3: Daudi cell lysate
  • ICC staining of IKK alpha + IKK beta in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of IKK alpha + IKK beta in B16F1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of IKK alpha + IKK beta in C2C12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of IKK alpha + IKK beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lane 3: Daudi cell lysate

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IKK alpha + IKK beta Monoclonal Antibody (ET1611-23)

Immunogen

Recombinant protein within human ikk beta aa 460-660.

Host

Rabbit

Positive Control

Hela cell lysate, A431 cell lysate, Daudi cell lysate, Hela, B16F1, C2C12.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN63-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

87 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

IKK alpha + IKK beta

SYNONYMS

CHUK antibody; Conserved helix loop helix ubiquitous kinase antibody; I kappa B kinase 1 antibody; I kappa B kinase 2 antibody; I Kappa B kinase alpha antibody; I Kappa B kinase beta antibody; IkB kinase alpha subunit antibody; IkBKA antibody; IkBKB antibody; IKK a kinase antibody; IKK alpha antibody; IKK beta antibody; IKK1 antibody; IKK2 antibody; IKKA antibody; IKKB antibody; IMD15 antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody; Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody; Inhibitor of nuclear factor kappa-B kinase subunit beta antibody; NFKBIKA antibody; NFKBIKB antibody; Nuclear factor NF kappa B inhibitor kinase beta antibody; Nuclear factor NFkappaB inhibitor kinase alpha antibody; TCF 16 antibody; TCF16 antibody; Transcription factor 16 antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Membrane raft.

FUNCTION

The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase a (IKKa), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IkB-a on Ser 32 and 36, the sites that trigger its degradation. IKKa appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKa is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKa, IKKb and IKKg (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

CITATIONS

  • Cai, Guodong et al.

    Mechanism and effects of Zearalenone on mouse T lymphocytes activation in vitro. | Ecotoxicology and Environmental Safety [2018]