PRODUCT CODE: ET1705-40

Recombinant IKB epsilon Monoclonal Antibody (ET1705-40)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

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+
Western blot analysis of IKB epsilon on different cell lysate using anti-IKB epsilon antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Line 1: THP-1 Line 2: Jurkat Line 3: Hela
  • Western blot analysis of IKB epsilon on different cell lysate using anti-IKB epsilon antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Line 1: THP-1 Line 2: Jurkat Line 3: Hela
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKB epsilon antibody. Counter stained with hematoxylin.
Western blot analysis of IKB epsilon on different cell lysate using anti-IKB epsilon antibody at 1/1,000 dilution.
Positive control:
Line 1: THP-1 Line 2: Jurkat Line 3: Hela

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IKB epsilon Monoclonal Antibody (ET1705-40)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1, Jurkat, Hela, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM62-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

IKB epsilon

SYNONYMS

I kappa B epsilon antibody; I-kappa-B-epsilon antibody; IkappaBepsilon antibody; IkB E antibody; IkB-E antibody; IkB-epsilon antibody; IKBE antibody; IKBE_HUMAN antibody; MGC72568 antibody; NF kappa B inhibitor epsilon antibody; NF kappa BIE antibody; NF-kappa-B inhibitor epsilon antibody; NF-kappa-BIE antibody; NFkappa BIE antibody; NFkappaB inhibitor epsilon antibody; NFKBIE antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon antibody; OTTHUMP00000016522 antibody; Slc35b2 antibody; solute carrier family 35, member B2 antibody

SEQUENCE SIMILARITIES

Belongs to the NF-kappa-B inhibitor family.

TISSUE SPECIFICITY

Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.

POST-TRANSLATIONAL MODIFICATION

Serine phosphorylated; followed by proteasome-dependent degradation.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. 

FUNCTION

On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.