PRODUCT CODE: ET1611-1

Recombinant IKB beta Monoclonal Antibody (ET1611-1)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of IKB beta on different lysates using anti-IKB beta antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Jurkat <br />
 Lane 2: Hela <br />
 Lane 3: THP-1
  • Western blot analysis of IKB beta on different lysates using anti-IKB beta antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Jurkat <br />
 Lane 2: Hela <br />
 Lane 3: THP-1
  • ICC staining IKB beta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IKB beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IKB beta antibody. Counter stained with hematoxylin.
Western blot analysis of IKB beta on different lysates using anti-IKB beta antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: THP-1

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IKB beta Monoclonal Antibody (ET1611-1)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat cell lysate, Hela cell lysate, THP-1 cell lysate, A549, MCF-7, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN0648

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

IKB beta

SYNONYMS

I kappa B beta antibody; I-kappa-B-beta antibody; IkappaBbeta antibody; IKB beta antibody; IkB-B antibody; IkB-beta antibody; IKBB antibody; IKBB_HUMAN antibody; IkBbeta antibody; NF kappa BIB antibody; NF-kappa-B inhibitor beta antibody; NF-kappa-BIB antibody; Nfkbib antibody; Thyroid receptor interacting protein 9 antibody; Thyroid receptor-interacting protein 9 antibody; TR interacting protein 9 antibody; TR-interacting protein 9 antibody; TRIP-9 antibody; TRIP9 antibody

SEQUENCE SIMILARITIES

Belongs to the NF-kappa-B inhibitor family.

TISSUE SPECIFICITY

Expressed in all tissues examined.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

On the basis of both functional and structural considerations, members of the IkB family of proteins can be divided into four groups. The first of these groups, IkB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to kB sites, suggesting that the IkB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IkB family is represented by a protein designated IkB-β. The third group of IkB proteins is represented by IkB-ε, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IkB family member, IkB-é, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.