PRODUCT CODE: ET1703-10

Recombinant IgA Monoclonal Antibody (ET1703-10)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of IgA on human plasma lysates using anti-IgA antibody at 1/1,000 dilution.
  • Western blot analysis of IgA on human plasma lysates using anti-IgA antibody at 1/1,000 dilution.
  • ICC staining IgA in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IgA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IgA antibody. Counter stained with hematoxylin.
Western blot analysis of IgA on human plasma lysates using anti-IgA antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IgA Monoclonal Antibody (ET1703-10)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human plasma lysates, HepG2, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-42

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

IgA

SYNONYMS

Hepatocellular carcinoma-associated protein TB6 antibody; Ig alpha 1 chain C region antibody; Ig alpha 2 chain C region antibody; IGHA antibody; IGHA1 antibody; IGHA2 antibody; Immunoglobulin heavy constant alpha 1 antibody; Immunoglobulin heavy constant alpha 2 A2m marker antibody; Immunoglobulin heavy constant alpha 2 antibody; PIgR antibody; Poly-Ig receptor antibody; polymeric immunoglobulin receptor antibody; polymeric immunoglobulin receptor Secretory component antibody

POST-TRANSLATIONAL MODIFICATION

3-Hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with alpha-1-microglobulin to form heterogeneous polycyclic chromophores including hydroxanthommatin. The chromophore reacts with accessible cysteines forming non-reducible thioether cross-links with Ig alpha-1 chain C region Cys-352.; N- and O-glycosylated. N-glycan at Asn-144: Hex5HexNAc4.

SUBCELLULAR LOCATION

Cell membrane, Secreted.

FUNCTION

Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins. The IgA heavy chain is an α-chain, and the light chains are either κ- or λ- chains. IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces.