PRODUCT CODE: ET1704-93

Recombinant IDH2 Monoclonal Antibody (ET1704-93)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of IDH2 on different cell lysate using anti-IDH2 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Line 1: Mouse heart <br />
  Line 2: Rat heart<br />
  Line 3: 293T
  • Western blot analysis of IDH2 on different cell lysate using anti-IDH2 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Line 1: Mouse heart <br />
  Line 2: Rat heart<br />
  Line 3: 293T
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IDH2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IDH2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-IDH2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-IDH2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-IDH2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with IDH2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of IDH2 on different cell lysate using anti-IDH2 antibody at 1/1,000 dilution.
Positive control:
Line 1: Mouse heart
Line 2: Rat heart
Line 3: 293T

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IDH2 Monoclonal Antibody (ET1704-93)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, mouse heart tissue lysate, rat heart tissue lysate, human liver tissue, human prostate tissue, MCF-7.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA55-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

IDH2

SYNONYMS

D2HGA2 antibody; ICD-M antibody; IDH antibody; IDH2 antibody; IDHM antibody; IDHP_HUMAN antibody; IDP antibody; IDPM antibody; Isocitrate dehydrogenase [NADP], mitochondrial antibody; Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody; mNADP-IDH antibody; NADP(+)-specific ICDH antibody; Oxalosuccinate decarboxylase antibody

SEQUENCE SIMILARITIES

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

POST-TRANSLATIONAL MODIFICATION

Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

IDH2 (isocitrate dehydrogenase 2 (NADP+), mitochondrial), also designated NADP+-specific ICDH; isocitrate dehydrogenase, mitochondrial; and oxalosuccinate decarboxylase, is a 452 amino acid enzyme encoded by the human gene IDH2. IDH2 belongs to the isocitrate and isopropylmalate dehydrogenases family and contains two nucleotide binding regions. IDH2 is involved in the reduction of NADP+ to NADPH and maintains the supply of glutathione (GSH) in mitochondria. It is believed to play a role in intermediary metabolism and energy production. IDH2 also tightly associates with the pyruvate dehydrogenase complex. IDH2 is found in the mitochondrion as a homodimer and can bind one magnesium or manganese ion per subunit.