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PRODUCT CODE: ET1705-49

Recombinant Id1 Monoclonal Antibody (ET1705-49)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of Id1 on 293T cell using anti-Id1 antibody at 1/1,000 dilution.
  • Western blot analysis of Id1 on 293T cell using anti-Id1 antibody at 1/1,000 dilution.
  • Western blot analysis of Id1 on different cells lysatesusing anti-Id1 antibody at 1/500 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2:HepG2
  • ICC staining Id1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Id1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Id1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Id1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Id1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Id1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Id1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Id1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Id1 on 293T cell using anti-Id1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Id1 Monoclonal Antibody (ET1705-49)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, HepG2, Hela, A549, human tonsil tissue, human liver tissue, human colon tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM92-13

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P41134

PROTEIN NAME

Id1

SYNONYMS

bHLHb24 antibody; Class B basic helix-loop-helix protein 24 antibody; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) antibody; DNA binding protein inhibitor ID 1 antibody; DNA binding protein inhibitor ID1 antibody; DNA-binding protein inhibitor ID-1 antibody; Dominant negative helix loop helix protein antibody; ID 1 antibody; ID antibody; ID1 antibody; ID1_HUMAN antibody; Inhibitor of Differentiation 1 antibody; Inhibitor of DNA binding 1 antibody; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein antibody

DEVELOPMENTAL STAGE

Expression correlates with proliferation in some types of cells.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.