Positive control:
Lane 1: Rat spleen tissue
Lane 2: Rat heart tisse
Lane 3: PC-12
Lane 4: Mouse heart tissue
Lane 5: Hela
Applications
-
WB
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant HuR ELAVL1 Monoclonal Antibody (ET1705-81)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Rat spleen tissue, rat heart tissue, PC-12, Mouse heart tissue, Hela, Jurkat, rat lung tissue, human colon cancer tissue, human breast tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM96-33
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:100-1:500
-
FC
-
1:50-1:100
-
IP
-
1:10-1:50
TARGET
UNIPROT #
PROTEIN NAME
HuR ELAVL1
SYNONYMS
ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody; ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody; ELAV like 1 antibody; ELAV like RNA binding protein 1 antibody; ELAV-like protein 1 antibody; ELAV1 antibody; ELAV1_HUMAN antibody; Elavl1 antibody; Embryonic lethal abnormal vision drosophila homolog like 1 antibody; Hu Antigen R antibody; Hu-antigen R antibody; Hua antibody; HuR antibody; MelG antibody
SEQUENCE SIMILARITIES
Belongs to the RRM elav family.
TISSUE SPECIFICITY
Ubiquitous. Detected in brain, liver, thymus and muscle.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by MAPKAPK2. Phosphorylated by PRKCD.; Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA.