PRODUCT CODE: ET1705-81

Recombinant HuR ELAVL1 Monoclonal Antibody (ET1705-81)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of HuR/ELAVL1 on different lysates using anti-HuR/ELAVL1 antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: Rat spleen tissue<br />
Lane 2: Rat heart tisse<br />
Lane 3: PC-12<br />
Lane 4: Mouse heart tissue<br />
Lane 5: Hela
  • Western blot analysis of HuR/ELAVL1 on different lysates using anti-HuR/ELAVL1 antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: Rat spleen tissue<br />
Lane 2: Rat heart tisse<br />
Lane 3: PC-12<br />
Lane 4: Mouse heart tissue<br />
Lane 5: Hela
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with HuR/ELAVL1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of HuR/ELAVL1 on different lysates using anti-HuR/ELAVL1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat spleen tissue
Lane 2: Rat heart tisse
Lane 3: PC-12
Lane 4: Mouse heart tissue
Lane 5: Hela

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HuR ELAVL1 Monoclonal Antibody (ET1705-81)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat spleen tissue, rat heart tissue, PC-12, Mouse heart tissue, Hela, Jurkat, rat lung tissue, human colon cancer tissue, human breast tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM96-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

ELAV-like protein 1

GENE NAME

ELAVL1

SYNONYMS

HuR, ELAVL1, HUR

SEQUENCE SIMILARITIES

Belongs to the RRM elav family.

TISSUE SPECIFICITY

Ubiquitous. Detected in brain, liver, thymus and muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by MAPKAPK2. Phosphorylated by PRKCD.; Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Cytoplasm, Stress granule.

FUNCTION

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR.