PRODUCT CODE: ET1702-81

Recombinant Human IgM Monoclonal Antibody (ET1702-81)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of Human IgM on human plasma lysates using anti-Human IgM antibody at 1/1,000 dilution.
  • Western blot analysis of Human IgM on human plasma lysates using anti-Human IgM antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Human IgM antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Human IgM antibody. Counter stained with hematoxylin.
Western blot analysis of Human IgM on human plasma lysates using anti-Human IgM antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Human IgM Monoclonal Antibody (ET1702-81)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human plasma tissue, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF84-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Human IgM

SYNONYMS

Immunoglobin heavy chain constant region mu edit item name - Immunoglobin heavy chain mu constant region antibody; Immunoglobin heavy chain constant region mu antibody; AGM1 antibody; Constant region of heavy chain of IgM antibody; DKFZp686I15196 antibody; DKFZp686I15212 antibody; FLJ00385 antibody; Ig mu chain C region antibody; IGHM antibody; IgM heavy chain constant region antibody; Immunoglobin heavy constant mu antibody; Immunoglobulin mu antibody; MGC104996 antibody; MGC52291 antibody; MU antibody; VH antibody

SUBCELLULAR LOCATION

Secreted, Cell membrane.

FUNCTION

Immunoglobulin M (IgM) is the largest circulating antibody molecule in humans. It consists of a heavy chain (?-chain) and a light chain (κ- or λ-chain), as well as 5 base units and 10 binding sites, though it cannot bind all 10 simultaneously because of steric hindrance. IgM chain C refers to the constant region of the IgM heavy chain that is involved in immune regulation. IgM forms polymers by covalently linking multiple immunoglobulins together with disulfide bonds. It normally exists as a pentamer, but occasionally as a hexamer. Because of its polymeric nature, IgM has high avidity, and it is especially effective at complement activation. Due to its large size, IgM does not diffuse well, and it is found in the interstitium in very low amounts. IgM is mainly found in serum; however, because of the J chain, it is also important as a secretory immunoglobulin. IgM is the first immunoglobulin expressed by mature B cells, and it normally appears early in the course of an infection and does not reappear after further exposure.