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PRODUCT CODE: ET1612-93

Recombinant HSPA14 Monoclonal Antibody (ET1612-93)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: K562 <br /> Lane 2: COS-1
  • Western blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: K562 <br /> Lane 2: COS-1
  • ICC staining HSPA14 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HSPA14 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HSPA14 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.
Western blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: COS-1

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HSPA14 Monoclonal Antibody (ET1612-93)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, COS-1, 293, 293T, RH-35, rat kidney tissue, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-48

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

Q0VDF9

PROTEIN NAME

Heat shock 70 kDa protein 14

GENE NAME

HSPA14

SYNONYMS

HSPA14

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 70 family.

SUBCELLULAR LOCATION

Cytoplasm, cytosol.

FUNCTION

Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.