Positive control:
Lane 1: K562
Lane 2: COS-1
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant HSPA14 Monoclonal Antibody (ET1612-93)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
K562, COS-1, 293, 293T, RH-35, rat kidney tissue, human kidney tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD08-48
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
55 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
HSPA14
SYNONYMS
Heat shock 70 kDa protein 14 antibody; Heat shock 70kDa protein 14 antibody; Heat shock protein HSP60 antibody; Heat shock protein hsp70 related protein antibody; HSP60 antibody; HSP70 4 antibody; HSP70 like protein 1 antibody; HSP70-like protein 1 antibody; HSP70L1 antibody; HSP7E_HUMAN antibody; HSPA 14 antibody; HSPA14 antibody; MGC131990 antibody
SEQUENCE SIMILARITIES
Belongs to the heat shock protein 70 family.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, the transportation of nascent poly-peptide chains across cellular membranes and the regulation of protein folding. HSPA14 (heat shock 70kDa protein 14), also known as HSP70-4 or HSP70L1, is a 509 amino acid novel HSP protein derived from human dendritic cells. Belonging to the heat shock protein 70 family, HSPA14 is thought to promote dendritic cell maturation. It is also suggested that HSPA14 stimulates secretion of the proinflammatory cytokines interleukin 12p70 (IL-12p70), IL-1β, TNFα, and the chemokines IP-10, MIP-1α, MIP-1β, and normal T cell expressed and secreted (RANTES).