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PRODUCT CODE: ET1612-93

Recombinant HSPA14 Monoclonal Antibody (ET1612-93)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: K562 <br /> Lane 2: COS-1
  • Western blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: K562 <br /> Lane 2: COS-1
  • ICC staining HSPA14 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HSPA14 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HSPA14 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.
Western blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: COS-1

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HSPA14 Monoclonal Antibody (ET1612-93)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, COS-1, 293, 293T, RH-35, rat kidney tissue, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-48

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

Q0VDF9

PROTEIN NAME

HSPA14

SYNONYMS

Heat shock 70 kDa protein 14 antibody; Heat shock 70kDa protein 14 antibody; Heat shock protein HSP60 antibody; Heat shock protein hsp70 related protein antibody; HSP60 antibody; HSP70 4 antibody; HSP70 like protein 1 antibody; HSP70-like protein 1 antibody; HSP70L1 antibody; HSP7E_HUMAN antibody; HSPA 14 antibody; HSPA14 antibody; MGC131990 antibody

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 70 family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, the transportation of nascent poly-peptide chains across cellular membranes and the regulation of protein folding. HSPA14 (heat shock 70kDa protein 14), also known as HSP70-4 or HSP70L1, is a 509 amino acid novel HSP protein derived from human dendritic cells. Belonging to the heat shock protein 70 family, HSPA14 is thought to promote dendritic cell maturation. It is also suggested that HSPA14 stimulates secretion of the proinflammatory cytokines interleukin 12p70 (IL-12p70), IL-1β, TNFα, and the chemokines IP-10, MIP-1α, MIP-1β, and normal T cell expressed and secreted (RANTES).