PRODUCT CODE: ET1612-54

Recombinant Hsp75 Monoclonal Antibody (ET1612-54)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

Western blot analysis of Hsp75 on different lysates using anti-Hsp75 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: 293T
  • Western blot analysis of Hsp75 on different lysates using anti-Hsp75 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: 293T
Western blot analysis of Hsp75 on different lysates using anti-Hsp75 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T

Applications

  • WB

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Hsp75 Monoclonal Antibody (ET1612-54)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-71

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Heat shock protein 75 kDa, mitochondrial

GENE NAME

TRAP1

SYNONYMS

HSP 75, TRAP-1, TRAP1

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 90 family.

TISSUE SPECIFICITY

Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is highly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors.

SUBCELLULAR LOCATION

Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix.

FUNCTION

Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.