PRODUCT CODE: ET1701-11

Recombinant Hsp22 Monoclonal Antibody (ET1701-11)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Hsp22 on different lysates using anti-Hsp22 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HepG2 <br />
Lane 2: Mouse skeletal muscle
  • Western blot analysis of Hsp22 on different lysates using anti-Hsp22 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HepG2 <br />
Lane 2: Mouse skeletal muscle
  • ICC staining Hsp22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Hsp22 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Hsp22 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hsp22 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Hsp22 antibody. Counter stained with hematoxylin.
Western blot analysis of Hsp22 on different lysates using anti-Hsp22 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Mouse skeletal muscle

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Hsp22 Monoclonal Antibody (ET1701-11)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, SH-SY-5Y, Hela, HepG2, rat skeletal muscle tissue, mouse skeletal muscle tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ08-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Heat shock protein beta-8

GENE NAME

HSPB8

SYNONYMS

HspB8

SEQUENCE SIMILARITIES

Belongs to the small heat shock protein (HSP20) family.

TISSUE SPECIFICITY

Predominantly expressed in skeletal muscle and heart.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Note=Translocates to nuclear foci during heat shock.

FUNCTION

Displays temperature-dependent chaperone activity.