PRODUCT CODE: ET1612-81

Recombinant Hsp20 Monoclonal Antibody (ET1612-81)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Human skeletal muscle <br />
 Lane 2: Mouse skeletal muscle
  • Western blot analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Human skeletal muscle <br />
 Lane 2: Mouse skeletal muscle
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Hsp20 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human skeletal muscle
Lane 2: Mouse skeletal muscle

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Hsp20 Monoclonal Antibody (ET1612-81)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human skeletal muscle tissue, mouse skeletal muscle tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD086-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

17 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P:1:50-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Hsp20

SYNONYMS

epididymis luminal protein 55 antibody; FLJ32389 antibody; Heat shock 20 kDa like protein p20 antibody; Heat shock 20 kDa-like protein p20 antibody; Heat shock protein alpha crystallin related B6 antibody; Heat shock protein beta 6 antibody; Heat shock protein beta-6 antibody; Heat shock protein, 20-KD antibody; Heat-shock 27-KD protein 6 antibody; HEL55 antibody; Hsp20 antibody; HspB6 antibody; HSPB6_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the small heat shock protein (HSP20) family.

POST-TRANSLATIONAL MODIFICATION

The N-terminus is blocked.; Phosphorylated at Ser-16 by PKA and probably PKD1K; required to protect cardiomyocytes from apoptosis.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27 (Heme Oxygenase 1), HSP 32 and HSP 40. HSP 20 occurs in two complex sizes, dimers and multimers. It is related to stress proteins and occurs most abundantly in skeletal muscle and heart. HSP 20 is considerably shorter at the C-terminus and less polar than other small heat shock proteins.