Applications
-
WB
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant HSD11B1 Monoclonal Antibody (ET1706-27)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Mouse liver tissue lysate, human liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU18-39
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
32 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
HSD11B1
SYNONYMS
11 beta HSD 1 antibody; 11 beta HSD1 antibody; 11 beta hydroxysteroid dehydrogenase 1 antibody; 11 DH antibody; 11-beta hydroxysteroid dehydrogenase, type 1 antibody; 11-beta-HSD1 antibody; 11-beta-hydroxysteroid dehydrogenase 1 antibody; 11-DH antibody; 11DH antibody; Corticosteroid 11 beta dehydrogenase isozyme 1 antibody; Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody; CORTRD2 antibody; DHI1_HUMAN antibody; HDL antibody; HSD 11 antibody; HSD11 antibody; HSD11B antibody; HSD11B1 antibody; HSD11L antibody; Hydroxysteroid (11 beta) dehydrogenase antibody; Hydroxysteroid (11 beta) dehydrogenase 1 antibody; MGC13539 antibody; SDR26C1 antibody; short chain dehydrogenase/reductase family 26C, member 1 antibody
SEQUENCE SIMILARITIES
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
TISSUE SPECIFICITY
Widely expressed. Highest expression in liver.
POST-TRANSLATIONAL MODIFICATION
Glycosylated.
SUBCELLULAR LOCATION
Endoplasmic reticulum, Membrane.
FUNCTION
HSD11B1(Corticosteroid 11-beta-dehydrogenase isozyme 1) is also named as HSD11, HSD11L,11-DH and 11-beta-HSD1.It is a microsomal enzyme, which acts mainly as a NADP(H)- dependent reductase converting inactive cortisone (E) to active cortisol (F), thus regulating intracellular F access to glucocorticoid receptor. This enzyme is widely expressed not only in liver and adipose tissue, but also in adrenal, ovary and decidua.Western blot analysis in human liver, using a specific11-beta-HSD1 antibody, showed two bands at approximately 34 and 68 kDa confrming the dimeric nature of 11-beta-HSD1 in liver and In omental adipose tissue, an additional band of 50 kDa was strongly expressed.