Recombinant HSD11B1 Monoclonal Antibody (ET1706-27)

Western blot analysis of HSD11B1 on mouse liver tissue lysate using anti-HSD11B1 antibody at 1/500 dilution.

Applications

WB
IHC-P

REACTIVITY

Human
Mouse
Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HSD11B1 Monoclonal Antibody (ET1706-27)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse liver tissue lysate, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU18-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

WB
1:500-1:2,000

IHC-P
1:50-1:200

TARGET

UNIPROT #

P28845

PROTEIN NAME

HSD11B1

SYNONYMS

11 beta HSD 1 antibody; 11 beta HSD1 antibody; 11 beta hydroxysteroid dehydrogenase 1 antibody; 11 DH antibody; 11-beta hydroxysteroid dehydrogenase, type 1 antibody; 11-beta-HSD1 antibody; 11-beta-hydroxysteroid dehydrogenase 1 antibody; 11-DH antibody; 11DH antibody; Corticosteroid 11 beta dehydrogenase isozyme 1 antibody; Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody; CORTRD2 antibody; DHI1_HUMAN antibody; HDL antibody; HSD 11 antibody; HSD11 antibody; HSD11B antibody; HSD11B1 antibody; HSD11L antibody; Hydroxysteroid (11 beta) dehydrogenase antibody; Hydroxysteroid (11 beta) dehydrogenase 1 antibody; MGC13539 antibody; SDR26C1 antibody; short chain dehydrogenase/reductase family 26C, member 1 antibody

SEQUENCE SIMILARITIES

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

TISSUE SPECIFICITY

Widely expressed. Highest expression in liver.

POST-TRANSLATIONAL MODIFICATION

Glycosylated.

SUBCELLULAR LOCATION

Endoplasmic reticulum, Membrane.

FUNCTION

HSD11B1(Corticosteroid 11-beta-dehydrogenase isozyme 1) is also named as HSD11, HSD11L,11-DH and 11-beta-HSD1.It is a microsomal enzyme, which acts mainly as a NADP(H)- dependent reductase converting inactive cortisone (E) to active cortisol (F), thus regulating intracellular F access to glucocorticoid receptor. This enzyme is widely expressed not only in liver and adipose tissue, but also in adrenal, ovary and decidua.Western blot analysis in human liver, using a specific11-beta-HSD1 antibody, showed two bands at approximately 34 and 68 kDa confrming the dimeric nature of 11-beta-HSD1 in liver and In omental adipose tissue, an additional band of 50 kDa was strongly expressed.