Recombinant HSD11B1 Monoclonal Antibody (ET1706-27)

Western blot analysis of HSD11B1 on mouse liver tissue lysate using anti-HSD11B1 antibody at 1/500 dilution.

Applications

WB
IHC-P

REACTIVITY

Human
Mouse
Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HSD11B1 Monoclonal Antibody (ET1706-27)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse liver tissue lysate, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU18-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

WB
1:500-1:2,000

IHC-P
1:50-1:200

TARGET

UNIPROT #

P28845

PROTEIN NAME

Corticosteroid 11-beta-dehydrogenase isozyme 1

GENE NAME

HSD11B1

SYNONYMS

11-DH, 11-beta-HSD1, HSD11B1, HSD11, HSD11L, SDR26C1

SEQUENCE SIMILARITIES

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

TISSUE SPECIFICITY

Widely expressed. Highest expression in liver.

POST-TRANSLATIONAL MODIFICATION

Glycosylated.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Single-pass type II membrane protein.

FUNCTION

Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity).