PRODUCT CODE: ET1706-08

Recombinant HPRT Monoclonal Antibody (ET1706-08)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Hela <br />
  Lane 2: A431<br />
  Lane 3: MCF-7 <br />
  Lane 4: 293<br />
  Lane 5: Rat kidney <br />
  Lane 6: Rat brain<br />
  Lane 7: Mouse testis <br />
  Lane 8: Mouse colon
  • Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Hela <br />
  Lane 2: A431<br />
  Lane 3: MCF-7 <br />
  Lane 4: 293<br />
  Lane 5: Rat kidney <br />
  Lane 6: Rat brain<br />
  Lane 7: Mouse testis <br />
  Lane 8: Mouse colon
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.
  • Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.
Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: A431
Lane 3: MCF-7
Lane 4: 293
Lane 5: Rat kidney
Lane 6: Rat brain
Lane 7: Mouse testis
Lane 8: Mouse colon

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HPRT Monoclonal Antibody (ET1706-08)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7 cell lysate, 293 cell lysate, Hela cell lysate, A431 cell lysate, rat kidney tissue lysate, rat tonsil brain lysate, mouse testis tissue lysate, mouse colon tissue lysate, zebrafish tissue lysates, rat brain tissue, human tonsil tissue, human colon carcinoma tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU03-26

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

HPRT

SYNONYMS

HGPRT antibody; HGPRTase antibody; HPRT 1 antibody; HPRT_HUMAN antibody; HPRT1 antibody; Hypoxanthine guanine phosphoribosyltransferase antibody; Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody; Hypoxanthine phosphoribosyltransferase 1 antibody; Hypoxanthine-guanine phosphoribosyltransferase antibody

SEQUENCE SIMILARITIES

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism.