PRODUCT CODE: ET1706-02

Recombinant HP1 gamma Monoclonal Antibody (ET1706-02)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of HP1 gamma on PC-12 (1) and MCF-7 (2) cell lysate using anti-HP1 gamma antibody at 1/500 dilution.
  • Western blot analysis of HP1 gamma on PC-12 (1) and MCF-7 (2) cell lysate using anti-HP1 gamma antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with HP1 gamma antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of HP1 gamma on PC-12 (1) and MCF-7 (2) cell lysate using anti-HP1 gamma antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HP1 gamma Monoclonal Antibody (ET1706-02)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, MCF-7, rat testis tissue, human tonsil tissue, human placenta tissue, mouse colon tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU81-36

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Chromobox protein homolog 3

GENE NAME

CBX3

SYNONYMS

HP1 gamma, CBX3

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

SUBCELLULAR LOCATION

Nucleus. Note=Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential).

FUNCTION

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs).