PRODUCT CODE: ET7107-10

Recombinant hnRNP U Monoclonal Antibody (ET7107-10)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of HnRNP U on different cell lysates using anti-HnRNP U at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: NIH-3T3 <br />
  Lane 2: SH-SY-5Y<br />
  Lane 3: K562
  • Western blot analysis of HnRNP U on different cell lysates using anti-HnRNP U at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: NIH-3T3 <br />
  Lane 2: SH-SY-5Y<br />
  Lane 3: K562
  • ICC staining HnRNP U in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HnRNP U in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HnRNP U in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HnRNP U antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HnRNP U antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-HnRNP U antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HnRNP U antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HnRNP U antibody. Counter stained with hematoxylin.
Western blot analysis of HnRNP U on different cell lysates using anti-HnRNP U at 1/500 dilution.
Positive control:
Lane 1: NIH-3T3
Lane 2: SH-SY-5Y
Lane 3: K562

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant hnRNP U Monoclonal Antibody (ET7107-10)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, HepG2, SH-SY-5Y, human tonsil tissue, human kidney tissue, human lung cancer tissue, mouse colon tissue, rat brain tissue, NIH-3T3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB23-43

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

120 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

hnRNP U

SYNONYMS

Heterogeneous nuclear ribonucleoprotein U antibody; hnRNP U antibody; hnRNP U protein antibody; HNRNPU antibody; hnRNPU protein antibody; HNRPU antibody; HNRPU_HUMAN antibody; p120 antibody; p120 nuclear protein antibody; pp120 antibody; SAF A antibody; SAF-A antibody; SAFA antibody; Scaffold attachment factor A antibody; U21.1 antibody

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Cleaved at Asp-100 by CASP3 during T-cell apoptosis, resulting in a loss of DNA- and chromatin-binding activities.; Extensively phosphorylated. Phosphorylated on Ser-59 by PLK1 and dephosphorylated by protein phosphatase 2A (PP2A) in mitosis.; Arg-739 is dimethylated, probably to asymmetric dimethylarginine (Ref.8). Arg-733 is dimethylated, probably to asymmetric dimethylarginine (By similarity).; Citrullinated by PADI4.

SUBCELLULAR LOCATION

Nucleus. Cytoskeleton.

FUNCTION

DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression. Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability. Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator. Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus.