Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH/3T3 cell lysate
Recombinant Rabbit monoclonal primary
Recombinant hnRNP K Monoclonal Antibody (ET1610-38)
Jurkat cell lysate, Hela cell lysate, NIH/3T3 cell lysate, Hela, HepG2, SKOV-3, human colon carcinoma tissue, mouse brain tissue, human breast carcinoma tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
CSBP antibody; dC stretch binding protein antibody; FLJ41122 antibody; Heterogeneous nuclear ribonucleoprotein K antibody; hnRNP K antibody; HNRNPK antibody; HNRPK antibody; HNRPK_HUMAN antibody; Transformation up regulated nuclear protein antibody; Transformation up-regulated nuclear protein antibody; Transformation upregulated nuclear protein antibody; TUNP antibody
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.; Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions.; Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination.; O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Nucleoplasm, Cytoplasm, podosome
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.