PRODUCT CODE: ET1611-2

Recombinant hnRNP C1+C2 Monoclonal Antibody (ET1611-2)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: MCF-7 <br />
 Lane 3: HepG2
  • Western blot analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: MCF-7 <br />
 Lane 3: HepG2
  • ICC staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with hnRNP C1+C2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: HepG2

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant hnRNP C1+C2 Monoclonal Antibody (ET1611-2)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

B16-F1, MCF-7, Hela, HepG2, mouse skin tissue, human breast carcinoma tissue, mouse placenta tissue, human kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN0652

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

hnRNP C1+C2

SYNONYMS

C1 antibody; C2 antibody; Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody; Heterogeneous nuclear ribonucleoprotein C antibody; Heterogeneous nuclear ribonucleoproteins C1/C2 antibody; HNRNP antibody; hnRNP C1 / hnRNP C2 antibody; hnRNP C1/C2 antibody; Hnrnpc antibody; HNRPC antibody; HNRPC_HUMAN antibody; MGC104306 antibody; MGC105117 antibody; MGC117353 antibody; MGC131677 antibody; Nuclear ribonucleoprotein particle C1 protein antibody; Nuclear ribonucleoprotein particle C2 protein antibody; SNRPC antibody

SEQUENCE SIMILARITIES

Belongs to the RRM HNRPC family. RALY subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.; Sumoylated. Sumoylation reduces affinity for mRNA.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and, in particular, the hnRNP A1 protein is one of the major pre-mRNA/mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however, some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, in-cluding hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the pre-mRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines.