Lane 1: Hela
Lane 2: MCF-7
Lane 3: HepG2
Recombinant Rabbit monoclonal primary
Recombinant hnRNP C1+C2 Monoclonal Antibody (ET1611-2)
B16-F1, MCF-7, Hela, HepG2, mouse skin tissue, human breast carcinoma tissue, mouse placenta tissue, human kidney tissue, mouse brain tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Heterogeneous nuclear ribonucleoproteins C1/C2
hnRNP C1/C2, HNRNPC
Belongs to the RRM HNRPC family. RALY subfamily.
Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.; Sumoylated. Sumoylation reduces affinity for mRNA.
Nucleus. Note=Component of ribonucleosomes.
Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing.