PRODUCT CODE: ET1706-51

Recombinant HLA-DQA1 Monoclonal Antibody (ET1706-51)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of HLA-DQA1 on different lysates using anti-HLA-DQA1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat lung <br />
 Lane 2: Rat skin <br />
 Lane 3: Mouse thymus <br />
 Lane 4: Mouse spleen<br />
 Lane 5: Raji<br />
  • Western blot analysis of HLA-DQA1 on different lysates using anti-HLA-DQA1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat lung <br />
 Lane 2: Rat skin <br />
 Lane 3: Mouse thymus <br />
 Lane 4: Mouse spleen<br />
 Lane 5: Raji<br />
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HLA-DQA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HLA-DQA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HLA-DQA1 antibody. Counter stained with hematoxylin.
Western blot analysis of HLA-DQA1 on different lysates using anti-HLA-DQA1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat lung
Lane 2: Rat skin
Lane 3: Mouse thymus
Lane 4: Mouse spleen
Lane 5: Raji

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HLA-DQA1 Monoclonal Antibody (ET1706-51)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat lung tissue, rat skin tissue lysate, mouse thymus tissue lysate, mouse spleen tissue lysate, Raji, human tonsil tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU17-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

HLA class II histocompatibility antigen, DQ alpha 1 chain

GENE NAME

HLA-DQA1

SYNONYMS

HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1

SEQUENCE SIMILARITIES

Belongs to the MHC class II family.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

FUNCTION

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.