PRODUCT CODE: ET1612-24

Recombinant Histone H1.0 Monoclonal Antibody (ET1612-24)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Histone H1.0 on human lung lysates using anti-Histone H1.0 antibody at 1/1,000 dilution.
  • Western blot analysis of Histone H1.0 on human lung lysates using anti-Histone H1.0 antibody at 1/1,000 dilution.
  • ICC staining Histone H1.0 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.
Western blot analysis of Histone H1.0 on human lung lysates using anti-Histone H1.0 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Histone H1.0 Monoclonal Antibody (ET1612-24)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, human tonsil tissue, human breast carcinoma tissue, human kidney tissue, mouse liver tissue, human pancreas tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD206-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Histone H1.0

GENE NAME

H1-0

SYNONYMS

H1F0

SEQUENCE SIMILARITIES

Belongs to the histone H1/H5 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Ser-17 in RNA edited version.; ADP-ribosylated on Ser-104 in response to DNA damage.

SUBCELLULAR LOCATION

Nucleus. Chromosome. Note=The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.

FUNCTION

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.