PRODUCT CODE: ET7107-32

Recombinant HIF-2 alpha Monoclonal Antibody (ET7107-32)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of HIF-2 alpha on SiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.
  • Western blot analysis of HIF-2 alpha on SiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HUVEC cells with HIF-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of HIF-2 alpha on SiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HIF-2 alpha Monoclonal Antibody (ET7107-32)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SiHa, rat lung tissue, human breast cancer tissue, human placenta tissue, mouse colon tissue, HUVEC.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB24-42

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

96 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

HIF-2 alpha

SYNONYMS

Basic helix loop helix PAS protein MOP2 antibody; Basic-helix-loop-helix-PAS protein MOP2 antibody; bHLHe73 antibody; Class E basic helix-loop-helix protein 73 antibody; ECYT4 antibody; Endothelial PAS domain containing protein 1 antibody; Endothelial pas domain protein 1 antibody; Endothelial PAS domain-containing protein 1 antibody; EPAS 1 antibody; EPAS-1 antibody; EPAS1 antibody; EPAS1_HUMAN antibody; HIF 1 alpha like factor antibody; HIF 2 alpha antibody; HIF-1-alpha-like factor antibody; HIF-2-alpha antibody; HIF2-alpha antibody; HIF2A antibody; HLF antibody; Hypoxia inducible factor 2 alpha antibody; Hypoxia inducible factor 2 alpha subunit antibody; Hypoxia-inducible factor 2-alpha antibody; Member of PAS protein 2 antibody; Member of pas superfamily 2 antibody; MOP 2 antibody; MOP2 antibody; PAS domain-containing protein 2 antibody; PASD2 antibody

TISSUE SPECIFICITY

Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

POST-TRANSLATIONAL MODIFICATION

In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity).; In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.; Phosphorylated on multiple sites in the CTAD.; The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.