PRODUCT CODE: ET1706-10

Recombinant Hemopexin Monoclonal Antibody (ET1706-10)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of Hemopexin on human plasma lysate using anti-Hemopexin antibody at 1/500 dilution.
  • Western blot analysis of Hemopexin on human plasma lysate using anti-Hemopexin antibody at 1/500 dilution.
  • ICC staining Hemopexin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Hemopexin in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Hemopexin in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Hemopexin in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Hemopexin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Hemopexin antibody. Counter stained with hematoxylin.
Western blot analysis of Hemopexin on human plasma lysate using anti-Hemopexin antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Hemopexin Monoclonal Antibody (ET1706-10)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human plasma lysate, HepG2, LO2, LOVO, SK-Br-3, human liver tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU83-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Hemopexin

SYNONYMS

Beta 1B glycoprotein antibody; Beta-1B-glycoprotein antibody; FLJ56652 antibody; HEMO antibody; HEMO_HUMAN antibody; Hemopexin antibody; Hpx antibody; HX antibody

SEQUENCE SIMILARITIES

Belongs to the hemopexin family.

TISSUE SPECIFICITY

Expressed by the liver and secreted in plasma.

POST-TRANSLATIONAL MODIFICATION

N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylation in the 30-40 region is minor compared to glycosylation at Thr-24 and Thr-29.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Hemopexin (also known as β 1B glycoprotein or HPX), a 462 amino acid protein, functions as a scavenger and transporter of toxic plasma heme, transporting heme to the liver for breakdown and iron recovery. Hemopexin cooperates with Albumin, Haptoglobin, and high and low density lipoproteins to trap toxic plasma heme, which occurs as the result of the degradation of hemoglobin, myoglobin and enzymes with heme prosthetic groups, and to ensure the clearance of toxic heme from the plasma. After releasing the heme molecule, the free Hemopexin returns to circulation. It is expressed by the liver and is secreted in plasma. Hemopexin may play a role in the maintenance of metal ion homeostasis. It binds the following metal ions in order of highest to lowest affinity: nickel, copper, cobalt, zinc and manganese. Hemopexin can also act as a toxic protease that leads to proteinuria and glomerular alterations, which are characteristics of minimal changes disease (MCD), a common cause of nephrotic syndrome.