Positive control:
Lane 1: SW480 cell lysate
Lane 2: SKOV-3 cell lysate
Lane 2: HepG2 cell lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant HE4 Monoclonal Antibody (ET1702-61)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
SW480 cell lysate, SKOV-3 cell lysate, HepG2 cell lysate, SKOV-3, SW480, mouse lung tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF62-09
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
23 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
HE4
SYNONYMS
dJ461P17.6 antibody; EDDM4 antibody; epididymal protein 4 antibody; Epididymal secretory protein E4 antibody; Epididymis specific whey acidic protein type four disulfide core antibody; HE 4 antibody; Major epididymis specific protein E4 antibody; Major epididymis-specific protein E4 antibody; MGC57529 antibody; Putative protease inhibitor WAP5 antibody; WAP 5 antibody; WAP domain containing protein HE4 antibody; WAP domain containing protein HE4 V4 antibody; WAP four disulfide core domain 2 antibody; WAP four disulfide core domain protein 2 antibody; WAP four-disulfide core domain protein 2 antibody; WAP5 antibody; WFDC 2 antibody; WFDC2 antibody; WFDC2_HUMAN antibody
TISSUE SPECIFICITY
Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
HE4 (whey acidic protein (WAP)-type four-disulfide core-2, WFDC2) is a small secretory protein that may influence sperm maturation. HE4 gene expression is high in pulmonary epithelial cells and in some ovarian cancers. HE4 protein has a WAP motif that contains eight cysteines forming four disulfide bonds at the core of the protein. The WAP motif functions as a protease inhibitor in many of the family members that contain them.