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PRODUCT CODE: ET1701-12

Recombinant HDAC8 Monoclonal Antibody (ET1701-12)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

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Western blot analysis of HDAC8 on different lysates using anti-HDAC8 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: K562
  • Western blot analysis of HDAC8 on different lysates using anti-HDAC8 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: K562
  • ICC staining HDAC8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HDAC8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HDAC8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with HDAC8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of HDAC8 on different lysates using anti-HDAC8 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: K562

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HDAC8 Monoclonal Antibody (ET1701-12)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, K562 cell lysate, Hela, A549, HepG2, human lung carcinoma tissue, human kidney tissue, human pancreas tissue, human lung tissue, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0845

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q9BY41

PROTEIN NAME

HDAC8

SYNONYMS

CDA07 antibody; CDLS5 antibody; HD 8 antibody; HD8 antibody; HDAC 8 antibody; Hdac8 antibody; HDAC8_HUMAN antibody; HDACL 1 antibody; HDACL1 antibody; Histone deacetylase 8 antibody; Histone deacetylase like 1 antibody; MRXS6 antibody; RPD 3 antibody; RPD3 antibody; WTS antibody

SEQUENCE SIMILARITIES

Belongs to the histone deacetylase family. HD type 1 subfamily.

TISSUE SPECIFICITY

Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.