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PRODUCT CODE: ET1612-51

Recombinant HDAC4 Monoclonal Antibody (ET1612-51)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

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Western blot analysis of HDAC4 on different lysates using anti-HDAC4 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: HepG2
  • Western blot analysis of HDAC4 on different lysates using anti-HDAC4 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: HepG2
  • ICC staining HDAC4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HDAC4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HDAC4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of HDAC4 on different lysates using anti-HDAC4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant HDAC4 Monoclonal Antibody (ET1612-51)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-35

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

120 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

P56524

PROTEIN NAME

HDAC4

SYNONYMS

AHO3 antibody; BDMR antibody; EC 3.5.1.98 antibody; HA6116 antibody; HD 4 antibody; HD4 antibody; HDAC 4 antibody; HDAC A antibody; HDAC4 antibody; HDAC4_HUMAN antibody; HDACA antibody; Histone deacetylase 4 antibody; Histone Deacetylase A antibody; KIAA0288 antibody

SEQUENCE SIMILARITIES

Belongs to the histone deacetylase family. HD type 2 subfamily.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350, within the PxLPxI/L motif, impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3.; Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, p300/CBP, PCAF (p300/CBPassociated factor), HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

CITATIONS

  • Wang, Hao et al.

    Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells. | Oncology Letters [2019]