PRODUCT CODE: ET1703-24

Recombinant Haptoglobin Monoclonal Antibody (ET1703-24)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Haptoglobin on different cells lysates using anti-Haptoglobin antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: HepG2
  • Western blot analysis of Haptoglobin on different cells lysates using anti-Haptoglobin antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: HepG2
  • ICC staining Haptoglobin in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Haptoglobin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Haptoglobin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.
Western blot analysis of Haptoglobin on different cells lysates using anti-Haptoglobin antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Haptoglobin Monoclonal Antibody (ET1703-24)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, HepG2 cell lysate, A549, Hela, HepG2, human liver tissue, human lung tissue, mouse liver tissue, mouse lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-79

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

45/38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Haptoglobin

SYNONYMS

Binding peptide antibody; BP antibody; Haptoglobin alpha chain antibody; Haptoglobin alpha(1S) beta antibody; Haptoglobin alpha(2FS) beta antibody; Haptoglobin beta chain antibody; Haptoglobin, alpha polypeptide antibody; Haptoglobin, beta polypeptide antibody; HP antibody; HP2 ALPHA2 antibody; HP2ALPHA2 antibody; HPA1S antibody; HPT antibody; HPT_HUMAN antibody; MGC111141 antibody; Zonulin antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase S1 family.

TISSUE SPECIFICITY

Expressed by the liver and secreted in plasma.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Haptoglobin (Hp) is a blood plasma protein that functions to bind free Hemoglobin that has been released from erythrocytes, thereby inhibiting its oxidative activity. During this process, Haptoglobin sequesters the iron within Hemoglobin, preventing iron-utilizing bacteria from benefitting from hemolysis. This function suggests that Haptoglobin concentrations may increase in response to inflammation. The resulting Haptoglobin-Hemoglobin complex is then removed by the reticulo-endothelial system. Due to cleavage of a common precursor protein during protein synthesis, Haptoglobin consists of two α and two β chains, connected by disulfide bridges. In human, Haptoglobin exists in two allelic forms designated Haptoglogin 1 (Hp1) and Haptoglobin 2 (Hp2), where Hp2 is the result of a partial Hp1 gene duplication. There are three known phenotypes of human Haptoglobin: Hp1-1, Hp2-1 and Hp2-2, which may be associated with diabetes and cardiovascular disease pathology and a susceptibility to Parkinson's and Crohn's disease. Haptoglobin levels are useful in diagnosing hemolytic anemia, the abnormal breakdown of red blood cells. Haptoglobin is expressed in mammalian hepatocytes as well as other tissues such as skin, lung and kidney.

CITATIONS

  • Zheng, Xi et al.

    A circulating extracellular vesicles-based novel screening tool for colorectal cancer revealed by shotgun and data-independent acquisition mass spectrometry. | Journal of Extracellular Vesicles [2020]