PRODUCT CODE: ET1611-59

Recombinant Glycogen synthase Monoclonal Antibody (ET1611-59)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Glycogen synthase on Hela lysates using anti- Glycogen synthase antibody at 1/1,000 dilution.
  • Western blot analysis of Glycogen synthase on Hela lysates using anti- Glycogen synthase antibody at 1/1,000 dilution.
  • Western blot analysis of Glycogen synthase on A431 lysates using anti- Glycogen synthase antibody at 1/500 dilution.
  • ICC staining Glycogen synthase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glycogen synthase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glycogen synthase in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glycogen synthase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Glycogen synthase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293 cells with Glycogen synthase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Glycogen synthase on Hela lysates using anti- Glycogen synthase antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Glycogen synthase Monoclonal Antibody (ET1611-59)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, PC-3M, NIH-3T3, 293, mouse skeletal muscle tissue, mouse prostate tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN75-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

84 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Glycogen [starch] synthase, muscle

GENE NAME

GYS1

SYNONYMS

GYS1

SEQUENCE SIMILARITIES

Belongs to the glycosyltransferase 3 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity). Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.

FUNCTION

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.