PRODUCT CODE: ET1702-09

Recombinant Glutathione Peroxidase 1 Monoclonal Antibody (ET1702-09)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Rat

Western blot analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution.
  • Western blot analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution.
  • ICC staining Glutathione Peroxidase 1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glutathione Peroxidase 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glutathione Peroxidase 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
Western blot analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Glutathione Peroxidase 1 Monoclonal Antibody (ET1702-09)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1, 293, HepG2, 293T, human liver tissue, human tonsil tissue, human breast carcinoma tissue, human kidney tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0944

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Glutathione peroxidase 1

GENE NAME

GPX1

SYNONYMS

GPx-1, GSHPx-1, GPX1

SEQUENCE SIMILARITIES

Belongs to the glutathione peroxidase family.

TISSUE SPECIFICITY

Expressed in platelets (at protein level).

POST-TRANSLATIONAL MODIFICATION

During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism.