Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
REACTIVITY
-
Human
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Glutathione Peroxidase 1 Monoclonal Antibody (ET1702-09)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
THP-1, 293, HepG2, 293T, human liver tissue, human tonsil tissue, human breast carcinoma tissue, human kidney tissue, mouse colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF0944
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
22 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Glutathione Peroxidase 1
SYNONYMS
AL033363 antibody; Cellular glutathione peroxidase antibody; Glutathione peroxidase 1 antibody; Glutathione peroxidase antibody; GPx 1 antibody; GPx-1 antibody; GPX1 antibody; GPX1_HUMAN antibody; GPXD antibody; GSHPx-1 antibody; GSHPX1 antibody; MGC14399 antibody; MGC88245 antibody
SEQUENCE SIMILARITIES
Belongs to the glutathione peroxidase family.
TISSUE SPECIFICITY
Expressed in platelets (at protein level).
POST-TRANSLATIONAL MODIFICATION
During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers. GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis.