PRODUCT CODE: ET1702-09

Recombinant Glutathione Peroxidase 1 Monoclonal Antibody (ET1702-09)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Rat

Western blot analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution.
  • Western blot analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution.
  • ICC staining Glutathione Peroxidase 1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glutathione Peroxidase 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glutathione Peroxidase 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
Western blot analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Glutathione Peroxidase 1 Monoclonal Antibody (ET1702-09)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1, 293, HepG2, 293T, human liver tissue, human tonsil tissue, human breast carcinoma tissue, human kidney tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0944

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Glutathione Peroxidase 1

SYNONYMS

AL033363 antibody; Cellular glutathione peroxidase antibody; Glutathione peroxidase 1 antibody; Glutathione peroxidase antibody; GPx 1 antibody; GPx-1 antibody; GPX1 antibody; GPX1_HUMAN antibody; GPXD antibody; GSHPx-1 antibody; GSHPX1 antibody; MGC14399 antibody; MGC88245 antibody

SEQUENCE SIMILARITIES

Belongs to the glutathione peroxidase family.

TISSUE SPECIFICITY

Expressed in platelets (at protein level).

POST-TRANSLATIONAL MODIFICATION

During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers. GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis.