PRODUCT CODE: ET1611-5

Recombinant Glutaminase Monoclonal Antibody (ET1611-5)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: 293
  • Western blot analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: 293
  • ICC staining Glutaminase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glutaminase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.
Western blot analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Glutaminase Monoclonal Antibody (ET1611-5)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293, Hela, HepG2, human tonsil tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN68-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

65 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Glutaminase kidney isoform, mitochondrial

GENE NAME

GLS

SYNONYMS

GLS

SEQUENCE SIMILARITIES

Belongs to the glutaminase family.

TISSUE SPECIFICITY

Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits.

SUBCELLULAR LOCATION

[Isoform 1]: Mitochondrion. Note=The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits.; [Glutaminase kidney isoform, mitochondrial 68 kDa chain]: Mitochondrion matrix. Note=Produced by the proteolytic processing of the 74-kDa cytosolic precursor.; [Glutaminase kidney isoform, mitochondrial 65 kDa chain]: Mitochondrion matrix. Note=Produced by the proteolytic processing of the 74-kDa cytosolic precursor.

FUNCTION

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain.; [Isoform 2]: Lacks catalytic activity.