Lane 1: Hela
Lane 2: 293
Recombinant Rabbit monoclonal primary
Recombinant Glutaminase Monoclonal Antibody (ET1611-5)
293, Hela, HepG2, human tonsil tissue, human kidney tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Glutaminase kidney isoform, mitochondrial
Belongs to the glutaminase family.
Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits.
[Isoform 1]: Mitochondrion. Note=The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits.; [Glutaminase kidney isoform, mitochondrial 68 kDa chain]: Mitochondrion matrix. Note=Produced by the proteolytic processing of the 74-kDa cytosolic precursor.; [Glutaminase kidney isoform, mitochondrial 65 kDa chain]: Mitochondrion matrix. Note=Produced by the proteolytic processing of the 74-kDa cytosolic precursor.
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain.; [Isoform 2]: Lacks catalytic activity.