PRODUCT CODE: ET1701-34

Recombinant GLUT2 Monoclonal Antibody (ET1701-34)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUT2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUT2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kideny tissue using anti-GLUT2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GLUT2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with GLUT2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUT2 antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GLUT2 Monoclonal Antibody (ET1701-34)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, Hela, HepG2, human liver tissue, human pancreas tissue, human kideny tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ20-21

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

57 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

GLUT2

SYNONYMS

liver antibody; Glucose Transporter 2 antibody; Glucose Transporter GLUT2 antibody; Glucose transporter type 2 antibody; Glucose transporter type 2 liver antibody; Glucose transporter, liver/islet antibody; GLUT-2 antibody; GLUT2 antibody; GTR2_HUMAN antibody; GTT2 antibody; SLC2A2 antibody; Solute carrier family 2 (facilitated glucose transporter) member 2 antibody; Solute carrier family 2 facilitated glucose transporter member 2 antibody; Solute carrier family 2, facilitated glucose transporter member 2 antibody

SEQUENCE SIMILARITIES

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

TISSUE SPECIFICITY

Liver, insulin-producing beta cell, small intestine and kidney.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. Glut1, Glut3 and Glut4 are high-affinity transporters, whereas Glut2 is a low-affinity transporter. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane and shows a much less dramatic translocation in response to insulin. Glut2 expression is seen in pancreatic beta cells, hepatocytes and basolateral membranes of intestinal and epithelial cells, while the highest expression of Glut3 has been found in neuronal tissue.