PRODUCT CODE: ET1704-59

Recombinant Glucose Transporter GLUT3 Monoclonal Antibody (ET1704-59)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Glucose Transporter GLUT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: rat heart tissue lysate<br />
 Lane 2: mouse heart tissue lysate<br />
 Lane 3: HepG2 cell lysate
  • Western blot analysis of Glucose Transporter GLUT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: rat heart tissue lysate<br />
 Lane 2: mouse heart tissue lysate<br />
 Lane 3: HepG2 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Glucose Transporter GLUT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human embryo tissue using anti-Glucose Transporter GLUT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Glucose Transporter GLUT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Glucose Transporter GLUT3 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-59, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Glucose Transporter GLUT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: rat heart tissue lysate
Lane 2: mouse heart tissue lysate
Lane 3: HepG2 cell lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Glucose Transporter GLUT3 Monoclonal Antibody (ET1704-59)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat heart tissue lysate, mouse heart tissue lysate, HepG2 cell lysate, human brain tissue, human embryo tissue, mouse brain tissue, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA50-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Glucose Transporter GLUT3

SYNONYMS

brain antibody; FLJ90380 antibody; Glucose transporter type 3 antibody; Glucose transporter type 3 brain antibody; GLUT 3 antibody; GLUT-3 antibody; GLUT3 antibody; GTR3_HUMAN antibody; Slc2a3 antibody; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody; Solute carrier family 2, facilitated glucose transporter member 3 antibody

SEQUENCE SIMILARITIES

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

TISSUE SPECIFICITY

Highly expressed in brain. Expressed in many tissues.

SUBCELLULAR LOCATION

Cell membrane. Perikaryon. Cell projection.

FUNCTION

Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. Glut1, Glut3 and Glut4 are high-affinity transporters, whereas Glut2 is a low-affinity transporter. In adipose and muscle tissue, Insulin stimulates a rapid and dramatic increase in glucose uptake, largely due to the redistribution of the Insulin-inducible glucose transporter, Glut4. In response to Insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane and shows a much less dramatic translocation in response to Insulin. Glut2 expression is seen in pancreatic b cells, hepatocytes and basolateral membranes of intestinal and epithelial cells, while the highest expression of Glut3 has been found in neuronal tissue.