PRODUCT CODE: ET7106-68

Recombinant GLO1 Monoclonal Antibody (ET7106-68)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse testis tissue <br />
  Lane 2: C2C12<br />
  Lane 3: 293 <br />
  Lane 4: Rat spleen tissue
  • Western blot analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse testis tissue <br />
  Lane 2: C2C12<br />
  Lane 3: 293 <br />
  Lane 4: Rat spleen tissue
  • ICC staining GLO1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GLO1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GLO1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLO1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-GLO1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with GLO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis tissue
Lane 2: C2C12
Lane 3: 293
Lane 4: Rat spleen tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GLO1 Monoclonal Antibody (ET7106-68)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse testis tissue lysate, C2C12, 293, rat spleen tissue lysate, 293T, HUVEC, SH-SY5Y, Hela, rat epididymis tissue, human thyroid gland tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU44-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

21 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

GLO1

SYNONYMS

Aldoketomutase antibody; glo1 antibody; GLOD1 antibody; Glx I antibody; GLYI antibody; glyoxalase domain containing 1 antibody; Glyoxalase I antibody; Ketone aldehyde mutase antibody; Ketone-aldehyde mutase antibody; Lactoyl glutathione lyase antibody; Lactoylglutathione lyase antibody; LGUL_HUMAN antibody; Methylglyoxalase antibody; S D lactoylglutathione methylglyoxal lyase antibody; S-D-lactoylglutathione methylglyoxal lyase antibody

SEQUENCE SIMILARITIES

Belongs to the glyoxalase I family.

POST-TRANSLATIONAL MODIFICATION

Glutathionylation at Cys-139 inhibits enzyme activity.; Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B.; Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

The glyoxal pathway plays a role in the detoxification of glucose degradation products (GDP). Glyoxalase I (GLO1), a member of the glyoxalase family, is effective in eliminating GDP. Overexpression or silencing of Glyoxalase I in mice brain suggests an association between Glyoxalase I and anxiety. Glyoxalase I has three isoforms generated from two alleles in the genome which forms two homodimers and one heterodimer, each subunit binding one zinc ion. Research demonstrates that GLO1 gene expression is induced in colon carcinoma. Both an insulin response element (IRE), and a zinc metal response element (MRE) in the promoter region of the GLO1 gene have been identified.