PRODUCT CODE: ET7106-68

Recombinant GLO1 Monoclonal Antibody (ET7106-68)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse testis tissue <br />
 Lane 2: C2C12<br />
 Lane 3: 293 <br />
 Lane 4: Rat spleen tissue<br />
  • Western blot analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse testis tissue <br />
 Lane 2: C2C12<br />
 Lane 3: 293 <br />
 Lane 4: Rat spleen tissue<br />
  • ICC staining GLO1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GLO1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GLO1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLO1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-GLO1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with GLO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis tissue
Lane 2: C2C12
Lane 3: 293
Lane 4: Rat spleen tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GLO1 Monoclonal Antibody (ET7106-68)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse testis tissue lysate, C2C12, 293, rat spleen tissue lysate, 293T, HUVEC, SH-SY5Y, Hela, rat epididymis tissue, human thyroid gland tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU44-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

21 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Lactoylglutathione lyase

GENE NAME

GLO1

SYNONYMS

Glx I, GLO1

SEQUENCE SIMILARITIES

Belongs to the glyoxalase I family.

POST-TRANSLATIONAL MODIFICATION

Glutathionylation at Cys-139 inhibits enzyme activity.; Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B.; Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B.

FUNCTION

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.