PRODUCT CODE: ET7106-91

Recombinant GIT1 Monoclonal Antibody (ET7106-91)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1/500 dilution.
  • Western blot analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GIT1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GIT1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-GIT1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GIT1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with GIT1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GIT1 Monoclonal Antibody (ET7106-91)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue, human colon cancer tissue, human pancreas tissue, mouse fallopian tubes tissue, A549.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU33-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

85 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

GIT1

SYNONYMS

ARF GAP GIT1 antibody; ARF GTPase activating protein GIT1 antibody; ARF GTPase-activating protein GIT1 antibody; CAT 1 antibody; CAT-1 antibody; CaT1 antibody; Cool associated and tyrosine phosphorylated protein 1 antibody; Cool-associated and tyrosine-phosphorylated protein 1 antibody; G protein coupled receptor kinase interacting ArfGAP 1 antibody; G protein coupled receptor kinase interactor 1 antibody; G protein-coupled receptor kinase-interactor 1 antibody; GIT1 antibody; GIT1_HUMAN antibody; GRK interacting protein 1 antibody; GRK-interacting protein 1 antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity).

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade. GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking. GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling.