PRODUCT CODE: ET7106-91

Recombinant GIT1 Monoclonal Antibody (ET7106-91)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1/500 dilution.
  • Western blot analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GIT1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GIT1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-GIT1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GIT1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with GIT1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GIT1 Monoclonal Antibody (ET7106-91)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue, human colon cancer tissue, human pancreas tissue, mouse fallopian tubes tissue, A549.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU33-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

85 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ARF GTPase-activating protein GIT1

GENE NAME

GIT1

SYNONYMS

ARF GAP GIT1, CAT-1, CAT1, GIT1

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity).

SUBCELLULAR LOCATION

Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.

FUNCTION

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity).