PRODUCT CODE: ET7107-45

Recombinant Gelsolin Monoclonal Antibody (ET7107-45)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse thymus <br />
  Lane 2: Mouse lung<br />
  Lane 3: THP-1 <br />
  Lane 4: HepG2
  • Western blot analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse thymus <br />
  Lane 2: Mouse lung<br />
  Lane 3: THP-1 <br />
  Lane 4: HepG2
  • ICC staining Gelsolin in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Gelsolin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of THP-1 cells with Gelsolin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse thymus
Lane 2: Mouse lung
Lane 3: THP-1
Lane 4: HepG2

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Gelsolin Monoclonal Antibody (ET7107-45)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human spleen tissue, human kidney tissue, mouse lung tissue, NIH-3T3, SiHa, mouse thymus tissue lysate, mouse lung tissue lysate, THP-1, HepG2, THP-1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB36-68

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

86 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Gelsolin

SYNONYMS

Actin depolymerizing factor antibody; Actin-depolymerizing factor antibody; ADF antibody; AGEL antibody; Brevin antibody; DKFZp313L0718 antibody; GELS_HUMAN antibody; Gelsolin antibody; Gsn antibody

SEQUENCE SIMILARITIES

Belongs to the villin/gelsolin family.

TISSUE SPECIFICITY

Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.

SUBCELLULAR LOCATION

Cytoskeleton. Secreted.

FUNCTION

Gelsolin (also known as brevin, Actin-depolymerizing factor or ADF), a protein of leukocytes, platelets and other cells, severs Actin filaments in the presence of submicromolar calcium, thereby isolating cytoplasmic Actin gels. A calcium-independent mechanism reverses the process. A Gelsolin variant with 23 more amino-terminal amino acids is a plasma component probably involved in the clearance of Actin, the most abundant human protein, from the circulation. It has been suggested that a single gene encodes both cell and plasma gelsolins. Gelsolin may be unique in that it is made for both secretion and intracytoplasmic location. Amino acid homology was identified between Gelsolin and the amyloid of the Finnish variety of amyloidosis. The amyloid in this disorder is antigenically and structurally related to Gelsolin. Gelsolin is the principal intracellular and extracellular Actin-severing protein. Gelsolin and Gc protein together constitute the extracellular Actin-scavenger system which prevents the toxic effects of Actin release into the extracellular space under circumstances of cell necrosis.