PRODUCT CODE: ET1704-46

Recombinant GDNF Monoclonal Antibody (ET1704-46)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GDNF on HepG2 cell using anti-GDNF antibody at 1/1,000 dilution.
  • Western blot analysis of GDNF on HepG2 cell using anti-GDNF antibody at 1/1,000 dilution.
  • Flow cytometric analysis of SH-SY5Y cells with GDNF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of GDNF on HepG2 cell using anti-GDNF antibody at 1/1,000 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GDNF Monoclonal Antibody (ET1704-46)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA93-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:200

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

GDNF

SYNONYMS

Astrocyte derived trophic factor antibody; Astrocyte derived trophic factor 1 antibody; Astrocyte-derived trophic factor antibody; ATF 1 antibody; ATF 2 antibody; Atf antibody; ATF1 antibody; ATF2 antibody; gdnf antibody; GDNF_HUMAN antibody; Glial cell derived neurotrophic factor antibody; Glial Cell Line Derived Neurotrophic Factor antibody; Glial cell line-derived neurotrophic factor antibody; Glial derived neurotrophic factor antibody; HFB1 GDNF antibody; hGDNF antibody; HSCR3 antibody

SEQUENCE SIMILARITIES

Belongs to the TGF-beta family. GDNF subfamily.

TISSUE SPECIFICITY

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Glial cell line-derived neurotrophic factor (GDNF) has been identified as a potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons. GDNF is a glycosylated, disulfide-bonded homodimer and is a distantly related member of the TGFβ superfamily of growth regulatory ligands. GDNF contains the seven conserved cysteine residues in the same relative spacing characteristic of all members of the TGFβ superfamily. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. On the basis of these findings, it has been suggested that GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.