Recombinant Rabbit monoclonal primary
Recombinant GCLC Monoclonal Antibody (ET1704-38)
A431, rat kidney tissue, human fetal kidney tissue, human fetal liver tissue, Jurkat.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
EC 188.8.131.52 antibody; Gamma ECS antibody; Gamma glutamylcysteine synthetase antibody; Gamma-ECS antibod; Gamma-glutamylcysteine synthetase antibody; GCL antibody; Gclc antibody; GCS antibody; GCS heavy chain antibody; GLCL antibody; GLCLC antibody; Glutamate cysteine ligase catalytic subunit antibody; Glutamate--cysteine ligase catalytic subunit antibody; GSH1_HUMAN antibody
Belongs to the glutamate--cysteine ligase type 3 family.
The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels.