PRODUCT CODE: ET1704-38

Recombinant GCLC Monoclonal Antibody (ET1704-38)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of GCLC on A431 cell using anti-GCLC antibody at 1/1,000 dilution.
  • Western blot analysis of GCLC on A431 cell using anti-GCLC antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GCLC antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue using anti-GCLC antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal liver tissue using anti-GCLC antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of GCLC on A431 cell using anti-GCLC antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GCLC Monoclonal Antibody (ET1704-38)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, rat kidney tissue, human fetal kidney tissue, human fetal liver tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA08-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

73 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

GCLC

SYNONYMS

EC 6.3.2.2 antibody; Gamma ECS antibody; Gamma glutamylcysteine synthetase antibody; Gamma-ECS antibod; Gamma-glutamylcysteine synthetase antibody; GCL antibody; Gclc antibody; GCS antibody; GCS heavy chain antibody; GLCL antibody; GLCLC antibody; Glutamate cysteine ligase catalytic subunit antibody; Glutamate--cysteine ligase catalytic subunit antibody; GSH1_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the glutamate--cysteine ligase type 3 family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels.