PRODUCT CODE: ET1704-41

Recombinant GATA1 Monoclonal Antibody (ET1704-41)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of GATA1 on K562 cell using anti-GATA1 antibody at 1/1,000 dilution.
  • Western blot analysis of GATA1 on K562 cell using anti-GATA1 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-GATA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GATA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GATA1 antibody. Counter stained with hematoxylin.
Western blot analysis of GATA1 on K562 cell using anti-GATA1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GATA1 Monoclonal Antibody (ET1704-41)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

K562, human liver tissue, human colon cancer tissue, human spleen tissue, human breast cancer tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA10-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Erythroid transcription factor

GENE NAME

GATA1

SYNONYMS

GATA-1, GF-1, GATA1, ERYF1, GF1

TISSUE SPECIFICITY

Erythrocytes.

POST-TRANSLATIONAL MODIFICATION

Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity).; Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity).; Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo (By similarity). Acetylated on Lys-233, Lys-245 Lys-246 by EP300.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.