PRODUCT CODE: ET1610-67

Recombinant GARS Monoclonal Antibody (ET1610-67)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GARS on Raji cells lysates using anti-GARS antibody at 1/1,000 dilution.
  • Western blot analysis of GARS on Raji cells lysates using anti-GARS antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-GARS antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-GARS antibody. Counter stained with hematoxylin.
Western blot analysis of GARS on Raji cells lysates using anti-GARS antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GARS Monoclonal Antibody (ET1610-67)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Raji, mouse colon tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC0651

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Glycine--tRNA ligase

GENE NAME

GARS1

SYNONYMS

AP-4-A synthetase, GlyRS, GARS

SEQUENCE SIMILARITIES

Belongs to the class-II aminoacyl-tRNA synthetase family.

TISSUE SPECIFICITY

Widely expressed, including in brain and spinal cord.

SUBCELLULAR LOCATION

Cytoplasm. Mitochondrion. Secreted.

FUNCTION

Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.