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PRODUCT CODE: ET7107-56

Recombinant Galectin 8 Monoclonal Antibody (ET7107-56)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Galectin 8 on mouse testis and rat testis tissue lysates using anti-Galectin 8 antibody at 1/500 dilution.
  • Western blot analysis of Galectin 8 on mouse testis and rat testis tissue lysates using anti-Galectin 8 antibody at 1/500 dilution.
  • ICC staining Galectin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Galectin 8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PC-3M cells with Galectin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Galectin 8 on mouse testis and rat testis tissue lysates using anti-Galectin 8 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Galectin 8 Monoclonal Antibody (ET7107-56)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse testis and rat testis tissue lysates, Human liver tissue, human colon cancer tissue, human kidney tissue, HepG2, A431, PC-3M.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB85-35

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:400-1:800

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

O00214

PROTEIN NAME

Galectin 8

SYNONYMS

Gal 8 antibody; Gal-8 antibody; Gal8 antibody; Galectin-8 antibody; galectin-8g antibody; Lectin galactoside binding soluble 8 antibody; LEG8_HUMAN antibody; LGAL S8 antibody; Lgals8 antibody; PCTA 1 antibody; PCTA-1 antibody; PCTA1 antibody; Po66 carbohydrate binding protein antibody; Po66 carbohydrate-binding protein antibody; Po66 CBP antibody; Po66-CBP antibody; Prostate carcinoma tumor antigen 1 antibody

TISSUE SPECIFICITY

Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.

SUBCELLULAR LOCATION

Cytosol.

FUNCTION

Several proteins have been identified as specific markers of prostate cancer, and they may be useful as diagnostic indicators. PSA, prostate specific antigen, is the classical indicator for transformed prostate tissue; however, in addition to being up-regulated in prostate cancer, PSA is also up-regulated in non-malignant conditions, such as benign prostatic hyperplasia prostate. Galectin-8, also known as prostate-specific membrane antigen (PCTA-1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family which mediates bothe cell-cell and cell-matrix interactions in a manner similar to the selectin subgroup of C-type lectins.