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PRODUCT CODE: ET1702-48

Recombinant Galectin 3 Monoclonal Antibody (ET1702-48)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Rat

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Western blot analysis of Galectin 3 on different lysates using anti-Galectin 3 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: NIH/3T3 <br /> Lane 3: THP-1
  • Western blot analysis of Galectin 3 on different lysates using anti-Galectin 3 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: NIH/3T3 <br /> Lane 3: THP-1
  • ICC staining Galectin 3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Galectin 3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Hela cells with Galectin 3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Galectin 3 on different lysates using anti-Galectin 3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: THP-1

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Galectin 3 Monoclonal Antibody (ET1702-48)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, THP-1, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF39-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

P17931

PROTEIN NAME

Galectin-3

GENE NAME

LGALS3

SYNONYMS

Gal-3, CBP 35, GALBP, LGALS3

TISSUE SPECIFICITY

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface.

FUNCTION

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.