Applications
-
WB
-
IP
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant GAD67 Monoclonal Antibody (ET1703-71)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM11-11
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
67 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IP
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
GAD67
SYNONYMS
67 kDa glutamic acid decarboxylase antibody; CPSQ1 antibody; DCE1 antibody; DCE1_HUMAN antibody; EC 4.1.1.15 antibody; FLJ45882 antibody; GAD 67 antibody; GAD antibody; GAD-67 antibody; GAD1 antibody; Glutamate decarboxylase 1 (brain, 67kDa) antibody; Glutamate decarboxylase 1 antibody; Glutamate decarboxylase 1 brain 67kD antibody; Glutamate decarboxylase 1 brain 67kDa antibody; Glutamate decarboxylase 67 kDa isoform antibody; Glutamate decarboxylase, brain, 67-KD antibody; OTTHUMP00000041055 antibody; SCP antibody
SEQUENCE SIMILARITIES
Belongs to the group II decarboxylase family.
TISSUE SPECIFICITY
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (g-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67, the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues.