PRODUCT CODE: ET7106-88

Recombinant GABA B Receptor 2 Monoclonal Antibody (ET7106-88)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GABA B Receptor 2 on mouse cerebellum tissue lysate using anti- GABA B Receptor 2 antibody at 1/500 dilution.
  • Western blot analysis of GABA B Receptor 2 on mouse cerebellum tissue lysate using anti- GABA B Receptor 2 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- GABA B Receptor 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti- GABA B Receptor 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- GABA B Receptor 2 antibody. Counter stained with hematoxylin.
  •  Flow cytometric analysis of SH-SY-5Y cells with GABA B Receptor 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of GABA B Receptor 2 on mouse cerebellum tissue lysate using anti- GABA B Receptor 2 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GABA B Receptor 2 Monoclonal Antibody (ET7106-88)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue, mouse brain tissue, mouse cerebellum tissue, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU31-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

106 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • ICC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Gamma-aminobutyric acid type B receptor subunit 2

GENE NAME

Gabbr2

SYNONYMS

GABA-B receptor 2, GABA-B-R2, GABA-BR2, GABABR2, Gb2, Gabbr2, Gm425, Gpr51

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Note=Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane (By similarity).

FUNCTION

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission (By similarity). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity).