PRODUCT CODE: ET1706-09

Recombinant Fragilis Monoclonal Antibody (ET1706-09)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Fragilis on different cell lysates using anti-Fragilis antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: HepG2<br />
 Lane 3: Hela<br />
  • Western blot analysis of Fragilis on different cell lysates using anti-Fragilis antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: HepG2<br />
 Lane 3: Hela<br />
  • ICC staining Fragilis in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fragilis in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fragilis in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with Fragilis antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Fragilis on different cell lysates using anti-Fragilis antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: Hela

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Fragilis Monoclonal Antibody (ET1706-09)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2, HUVEC, MCF-7, PC-3M, human liver cancer tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU73-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

15 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Interferon-induced transmembrane protein 3

GENE NAME

IFITM3

SYNONYMS

DSPA2b, IFITM3

SEQUENCE SIMILARITIES

Belongs to the CD225/Dispanin family.

POST-TRANSLATIONAL MODIFICATION

Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.; Not glycosylated.; Polyubiquitinated with both 'Lys-48' and 'Lys-63' linkages. Ubiquitination negatively regulates antiviral activity. Lys-24 is the most prevalent ubiquitination site.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type II membrane protein. Late endosome membrane; Single-pass type II membrane protein. Lysosome membrane; Single-pass type II membrane protein.

FUNCTION

IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state.