PRODUCT CODE: ET1612-66

Recombinant FOXO4 Monoclonal Antibody (ET1612-66)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of FOXO4 on human lung lysates using anti-FOXO4 antibody at 1/1,000 dilution.
  • Western blot analysis of FOXO4 on human lung lysates using anti-FOXO4 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-FOXO4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-FOXO4 antibody. Counter stained with hematoxylin.
Western blot analysis of FOXO4 on human lung lysates using anti-FOXO4 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant FOXO4 Monoclonal Antibody (ET1612-66)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human lung cancer tissue, human breast carcinoma tissue, human lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD0817

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54/48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

FOXO4

SYNONYMS

AFX antibody; AFX1 antibody; Afxh antibody; ALL1-fused gene from X chromosome antibody; Fork head domain transcription factor AFX1 antibody; Forkhead box O4 antibody; Forkhead box protein O4 antibody; FOXO 4 antibody; Foxo4 antibody; FOXO4_HUMAN antibody; MGC117660 antibody; MGC120490 antibody; Mixed lineage leukemia, translocated to, 7 antibody; MLLT7 antibody; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody; Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody; RGD1561201 antibody

TISSUE SPECIFICITY

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

POST-TRANSLATIONAL MODIFICATION

Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.; Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK.; Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

FKHR (for forkhead in rhabdomyosarcoma), FKHRL1, and AFX1 are members of a subfamily of the forkhead family of transcription factors. AFX1, also known as FoxO4, is expressed in a wide variety of tissues and, like other FKHR proteins, AFX1 contains a single forkhead domain and serine-proline-rich region, which mediate DNA binding. AFX1-mediated transcriptional activation is regulated by the serine/threonine kinase Akt1, which phosphorylates AFX1 and in turn, sequesters AFX1 in the cytosol, thereby blocking nuclear localization and DNA binding. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. Additionally, the t(x;11) translocation of the AFX1 gene, which involves the fusion of a serine-proline-rich sequence of AFX1 to the carboxy terminus of a truncated MLL, results in acute lymphocytic leukemia.