PRODUCT CODE: ET1703-76

Recombinant FOXA2 Monoclonal Antibody (ET1703-76)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

Western blot analysis of FOXA2 on HT-29 cells lysates using anti-FOXA2 antibody at 1/500 dilution.
  • Western blot analysis of FOXA2 on HT-29 cells lysates using anti-FOXA2 antibody at 1/500 dilution.
Western blot analysis of FOXA2 on HT-29 cells lysates using anti-FOXA2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant FOXA2 Monoclonal Antibody (ET1703-76)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HT29.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-64

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

FOXA2

SYNONYMS

Forkhead box A2 antibody; Forkhead box protein A2 antibody; FOX A2 antibody; foxa2 antibody; FOXA2_HUMAN antibody; Hepatic nuclear factor 3 beta antibody; Hepatocyte nuclear factor 3 antibody; Hepatocyte nuclear factor 3 beta antibody; Hepatocyte nuclear factor 3-beta antibody; HNF 3B antibody; HNF-3-beta antibody; HNF-3B antibody; HNF3B antibody; MGC19807 antibody; TCF 3B antibody; TCF-3B antibody; TCF3B antibody; Transcription factor 3B antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.