PRODUCT CODE: ET1602-44

Recombinant Firefly Luciferase Monoclonal Antibody (ET1602-44)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Firefly

Western blot analysis of Firefly Luciferase on luciferase recombinant protein lysate using anti-Firefly Luciferase antibody at 1/1,000 dilution.
  • Western blot analysis of Firefly Luciferase on luciferase recombinant protein lysate using anti-Firefly Luciferase antibody at 1/1,000 dilution.
Western blot analysis of Firefly Luciferase on luciferase recombinant protein lysate using anti-Firefly Luciferase antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Firefly

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Firefly Luciferase Monoclonal Antibody (ET1602-44)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Luciferase recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR03-86

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Firefly Luciferase

SYNONYMS

ec 1.13.12.7 antibody; Firefly antibody; Luciferase antibody; Luciferin 4 monooxygenase antibody

SEQUENCE SIMILARITIES

Belongs to the ATP-dependent AMP-binding enzyme family.

SUBCELLULAR LOCATION

Peroxisome.

FUNCTION

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.