PRODUCT CODE: ET1703-18

Recombinant FGF2 Monoclonal Antibody (ET1703-18)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

Flow cytometric analysis of Jurkat cells with FGF2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
  • Flow cytometric analysis of Jurkat cells with FGF2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Flow cytometric analysis of Jurkat cells with FGF2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant FGF2 Monoclonal Antibody (ET1703-18)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM28-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

  • IP

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

FGF2

SYNONYMS

Basic fibroblast growth factor antibody; Basic fibroblast growth factor bFGF antibody; BFGF antibody; FGF 2 antibody; FGF B antibody; FGF-2 antibody; Fgf2 antibody; FGF2 basic antibody; FGF2_HUMAN antibody; FGFB antibody; Fibroblast growth factor 2 (basic) antibody; Fibroblast growth factor 2 antibody; Fibroblast growth factor, basic antibody; HBGF 2 antibody; HBGF-2 antibody; HBGF2 antibody; HBGH 2 antibody; HBGH2 antibody; Heparin binding growth factor 2 precursor antibody; Heparin-binding growth factor 2 antibody; Prostatropin antibody

SEQUENCE SIMILARITIES

Belongs to the heparin-binding growth factors family.

TISSUE SPECIFICITY

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.; Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

SUBCELLULAR LOCATION

Secreted, Nucleus.

FUNCTION

Fibroblast growth factor-1 (FGF-1), also designated acidic FGF, and fibroblast growth factor-2 (FGF-2), also designated basic FGF, are members of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithe-lial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (hst/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10-FGF-23. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3.