PRODUCT CODE: ET7109-25

Recombinant Fbx32 Monoclonal Antibody (ET7109-25)

  • Recombinant

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution.
  • Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution.
  • ICC staining Fbx32 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fbx32 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Fbx32 Monoclonal Antibody (ET7109-25)

Immunogen

Recombinant protein corresponding to human fbx32 1-200aa.

Host

Rabbit

Positive Control

Mouse skeletal muscle tissue lysate, A431, L6.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE41-27

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

41 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

F-box only protein 32

GENE NAME

FBXO32

SYNONYMS

MAFbx, FBXO32

TISSUE SPECIFICITY

Specifically expressed in cardiac and skeletal muscle.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and the nucleus.

FUNCTION

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.