PRODUCT CODE: ET1705-18

Recombinant Fascin Monoclonal Antibody (ET1705-18)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution.<br />
Positive control:<br />
 Lane 1: SH-SY5Y <br />
 Lane 2: Human brain <br />
 Lane 3: Rat spleen <br />
 Lane 4: Mouse testes<br />
 Lane 5: Mouse brain<br />
 Lane 6: Mouse spleen<br />
  • Western blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution.<br />
Positive control:<br />
 Lane 1: SH-SY5Y <br />
 Lane 2: Human brain <br />
 Lane 3: Rat spleen <br />
 Lane 4: Mouse testes<br />
 Lane 5: Mouse brain<br />
 Lane 6: Mouse spleen<br />
  • ICC staining Fascin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fascin in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fascin in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fascin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fascin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fascin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Fascin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: Human brain
Lane 3: Rat spleen
Lane 4: Mouse testes
Lane 5: Mouse brain
Lane 6: Mouse spleen

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Fascin Monoclonal Antibody (ET1705-18)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY5Y, human brain tissue lysate, rat spleen tissue lysate, mouse testes tissue lysate, mouse brain tissue lysate, mouse spleen tissue lysate, Hela, SW480, rat brain tissue, human tonsil tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM12-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Fascin

GENE NAME

FSCN1

SYNONYMS

FSCN1, FAN1, HSN, SNL

SEQUENCE SIMILARITIES

Belongs to the fascin family.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-39 inhibits actin-binding. Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF.

SUBCELLULAR LOCATION

Cytoplasm, cytosol. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell projection, filopodium. Cell projection, invadopodium. Cell projection, microvillus. Cell junction. Note=Colocalized with RUFY3 and F-actin at filipodia of the axonal growth cone. Colocalized with DBN1 and F-actin at the transitional domain of the axonal growth cone (By similarity).

FUNCTION

Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.