PRODUCT CODE: ET1705-18

Recombinant Fascin Monoclonal Antibody (ET1705-18)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution.<br />
 Positive control:<br />
  Lane 1: SH-SY5Y <br />
  Lane 2: Human brain <br />
  Lane 3: Rat spleen <br />
  Lane 4: Mouse testes<br />
  Lane 5: Mouse brain<br />
  Lane 6: Mouse spleen
  • Western blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution.<br />
 Positive control:<br />
  Lane 1: SH-SY5Y <br />
  Lane 2: Human brain <br />
  Lane 3: Rat spleen <br />
  Lane 4: Mouse testes<br />
  Lane 5: Mouse brain<br />
  Lane 6: Mouse spleen
  • ICC staining Fascin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fascin in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fascin in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fascin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fascin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fascin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Fascin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: Human brain
Lane 3: Rat spleen
Lane 4: Mouse testes
Lane 5: Mouse brain
Lane 6: Mouse spleen

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Fascin Monoclonal Antibody (ET1705-18)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse testis tissue lysate, mouse spleen tissue lysate, SH-SY5Y cell lysate, human brain tissue lysate, Hela, SW480, SH-SY5Y, rat brain tissue, human kidney tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM12-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Fascin

SYNONYMS

55 kDa actin bundling protein antibody; 55 kDa actin-bundling protein antibody; Actin bundling protein antibody; actin bundling protein, 55-KD antibody; FAN 1 antibody; FAN1 antibody; Fascin 1 antibody; Fascin actin bundling protein 1 antibody; Fascin antibody; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody; Fascin homolog 1 antibody; Fascin, sea urchin, homolog of, 1 antibody; Fascin1 antibody; FLJ38511 antibody; FSCN 1 antibody; FSCN1 antibody; FSCN1_HUMAN antibody; HSN antibody; p55 antibody; Singed (Drosophila) like (sea urchin fascin homolog like) antibody; Singed drosophila homolog like antibody; Singed like (fascin homolog sea urchin) (Drosophila) antibody; Singed like (fascin homolog sea urchin) antibody; Singed like protein antibody; Singed, drosophila, homolog of antibody; Singed-like protein antibody; SNL antibody; Strongylocentrotus purpuratus antibody

SEQUENCE SIMILARITIES

Belongs to the fascin family.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-39 inhibits actin-binding. Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF.

SUBCELLULAR LOCATION

Cytoskeleton, stress fiber, cytosol, cell cortex, filopodium, invadopodium, microvillus, cell junction.

FUNCTION

Cell adhesion to extracellular matrix is an important physiological stimulus for organization of the Actin-based cytoskeleton. Adhesion to the matrix glycoprotein Thrombospondin 1 triggers the sustained formation of F-Actin microspikes that contain the Actin-bundling protein Fascin. These structures are also implicated in cell migration, which may be an important function of Thrombospondin 1 in tissue remodelling and wound repair. Fascin bundles Actin microfilaments within dynamic cellular structures such as microspikes, stress fibers and membrane ruffles. Fascin could serve as a prognostic factor for abnormal ovarian epithelial pathology and could be a novel target for the treatment of ovarian cancer. Fascin, an Actin-bundling protein, identifies dendritic cells in the blood and in tissues.